biorad s micropulser electroporator Search Results


micropulser  (Bio-Rad)
96
Bio-Rad micropulser
Micropulser, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/MicroPulser+Electroporator/pmc07324442-162-5-6
Average 96 stars, based on 1 article reviews
micropulser - by Bioz Stars, 2026-10
96/100 stars
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electroporation cuvette  (Bio-Rad)
96
Bio-Rad electroporation cuvette
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Gene+Pulser+%2FMicroPulser+Electroporation+Cuvettes/pmc04601864-65-19-21
Average 96 stars, based on 1 article reviews
electroporation cuvette - by Bioz Stars, 2026-10
96/100 stars
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cuvette  (Bio-Rad)
96
Bio-Rad cuvette
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Standard+Cuvette/bio_rxiv__448977-54-26-27
Average 96 stars, based on 1 article reviews
cuvette - by Bioz Stars, 2026-10
96/100 stars
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genepulser xcell electroporator  (Bio-Rad)
97
Bio-Rad genepulser xcell electroporator
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Genepulser Xcell Electroporator, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Gene+Pulser+Xcell+Total+System/pmc06387584-58-3-6
Average 97 stars, based on 1 article reviews
genepulser xcell electroporator - by Bioz Stars, 2026-10
97/100 stars
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cuvettes  (Bio-Rad)
96
Bio-Rad cuvettes
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Standard+Cuvettes/pmc05678006-119-21-22
Average 96 stars, based on 1 article reviews
cuvettes - by Bioz Stars, 2026-10
96/100 stars
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disposable electroporation cuvettes  (Avantor)
90
Avantor disposable electroporation cuvettes
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Disposable Electroporation Cuvettes, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/electroporation+cuvettes/bio_rxiv__2020__07__20__213264-238-18-19
Average 90 stars, based on 1 article reviews
disposable electroporation cuvettes - by Bioz Stars, 2026-10
90/100 stars
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gene pulser micropulser  (Bio-Rad)
93
Bio-Rad gene pulser micropulser
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Gene Pulser Micropulser, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Gene+Pulser%2FMicroPulser+Cuvette/bio_rxiv__2024__10__25__618925-319-24-26
Average 93 stars, based on 1 article reviews
gene pulser micropulser - by Bioz Stars, 2026-10
93/100 stars
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bucket for gene pulser  (OneLab Solutions)
99
OneLab Solutions bucket for gene pulser
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Bucket For Gene Pulser, supplied by OneLab Solutions, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Bucket+For+Gene+Pulser/custom%401652089%4010%2E1128%2Fjb%2E00030-20
Average 99 stars, based on 1 article reviews
bucket for gene pulser - by Bioz Stars, 2026-10
99/100 stars
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biorad xpulser  (Bio-Rad)
96
Bio-Rad biorad xpulser
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Biorad Xpulser, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Electroporation+Cuvettes+For+Gene+Pulser+%2FMicro+Pulser/pm27796471-47-20-20
Average 96 stars, based on 1 article reviews
biorad xpulser - by Bioz Stars, 2026-10
96/100 stars
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sucrose buffer  (Bio-Rad)
97
Bio-Rad sucrose buffer
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Sucrose Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Lysis+Buffer/pm38835975-60-16-23
Average 97 stars, based on 1 article reviews
sucrose buffer - by Bioz Stars, 2026-10
97/100 stars
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sterile electroporation cuvettes  (Genesee Scientific)
93
Genesee Scientific sterile electroporation cuvettes
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Sterile Electroporation Cuvettes, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/Electroporation+Cuvettes/pm37040165-56-16-19
Average 93 stars, based on 1 article reviews
sterile electroporation cuvettes - by Bioz Stars, 2026-10
93/100 stars
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gap cuvette  (PEQLAB)
90
PEQLAB gap cuvette
Physical map of the vectors and <t>electroporation</t> procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.
Gap Cuvette, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+s+micropulser+electroporator/gap+cuvette/pmc04894983__ncomms11667___s1-185-20-22
Average 90 stars, based on 1 article reviews
gap cuvette - by Bioz Stars, 2026-10
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Image Search Results


Physical map of the vectors and electroporation procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.

Journal: Cell Journal (Yakhteh)

Article Title: Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells

doi:

Figure Lengend Snippet: Physical map of the vectors and electroporation procedure. A. Both pVASA -EGFP and pOLIG2 -EGFP benefit from dual selection system including neomycin and puromycin. No difference was seen in transformation efficiency despite the different vectors’ sizes and B. Human embryonic stem cells (hESCs) were seeded onto 50 cm plates and grown in appropriate medium until they reached 70-80% confluency. Following incubation in Tryp/LE at 37˚C for 5 minutes the cells were singled. For electroporation, the cells were counted and resuspended in phosphate buffer saline (PBS) at a concentration of 1×10 6 , after which 700 μl of cell suspension was mixed with 20-60 μg of linear plasmid DNA in a sterile electroporation cuvette. The voltage varied from 240 to 300 V. Immediately after electroporation, the cells were removed from the cuvette and plated on three 10 cm diameter tissue culture dishes in complete medium. After 48 hours, the plates were washed twice with phosphate buffer saline (PBS), then replenished with complete medium. MEF; Mouse embryonic fibroblast.

Article Snippet: In brief, 600 μl of the previously singled cells that contained 10-60 μg linearized plasmid was transferred into the electroporation cuvette (BioRad, USA, #165-2088).

Techniques: Electroporation, Selection, Transformation Assay, Incubation, Saline, Concentration Assay, Suspension, Plasmid Preparation, Sterility

In vitro transfection of H5, H6 and human foreskin fibroblast (hFF) cell lines by lipofectamine and electroporation. A. A comparison of chemical and physical technique efficiencies for gene transformation confirmed a higher rate of transfection for electroporation in individual cell lines. The graph shows the averages of three independent experiments. Error bars represent the standard deviation. **; P<0.01 and B. Flow cytometric analysis of EGFP expression in three independent experiments. After 48 hours of gene delivery, we analyzed transient expression of EGFP by flow cytometry. hFF cells showed the highest percentage (27%) of expression when compared with the other cells. Interestingly, a comparison of the two different human embryonic stem cells (hESCs) demonstrated that Royan H5 exhibited greater transformation potential (approximately 17 vs. 10%).

Journal: Cell Journal (Yakhteh)

Article Title: Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells

doi:

Figure Lengend Snippet: In vitro transfection of H5, H6 and human foreskin fibroblast (hFF) cell lines by lipofectamine and electroporation. A. A comparison of chemical and physical technique efficiencies for gene transformation confirmed a higher rate of transfection for electroporation in individual cell lines. The graph shows the averages of three independent experiments. Error bars represent the standard deviation. **; P<0.01 and B. Flow cytometric analysis of EGFP expression in three independent experiments. After 48 hours of gene delivery, we analyzed transient expression of EGFP by flow cytometry. hFF cells showed the highest percentage (27%) of expression when compared with the other cells. Interestingly, a comparison of the two different human embryonic stem cells (hESCs) demonstrated that Royan H5 exhibited greater transformation potential (approximately 17 vs. 10%).

Article Snippet: In brief, 600 μl of the previously singled cells that contained 10-60 μg linearized plasmid was transferred into the electroporation cuvette (BioRad, USA, #165-2088).

Techniques: In Vitro, Transfection, Electroporation, Comparison, Transformation Assay, Standard Deviation, Expressing, Flow Cytometry

Transient expression of EGFP in Royan H6 and human foreskin fibroblast (hFF) cell lines. A, D. Bright-field images of human embryonic stem cells (hESCs) and hFF cells, B, E. Fluorescent images and C, F. Merged bright-field and fluorescent images. The pictures showed that hFF responded to electroporation more efficiently than hESCs.

Journal: Cell Journal (Yakhteh)

Article Title: Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells

doi:

Figure Lengend Snippet: Transient expression of EGFP in Royan H6 and human foreskin fibroblast (hFF) cell lines. A, D. Bright-field images of human embryonic stem cells (hESCs) and hFF cells, B, E. Fluorescent images and C, F. Merged bright-field and fluorescent images. The pictures showed that hFF responded to electroporation more efficiently than hESCs.

Article Snippet: In brief, 600 μl of the previously singled cells that contained 10-60 μg linearized plasmid was transferred into the electroporation cuvette (BioRad, USA, #165-2088).

Techniques: Expressing, Electroporation